Coding

Part:BBa_I10099:Design

Designed by: Alice Macdonald   Group: BE.109 2006 BE.109 Instructors   (2006-04-12)


coding region of FimH protein of E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 535
    Illegal AgeI site found at 838
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References

  • NCBI Protein Database (AAR27574) URL:http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&val=38885204#sequence_38885204. Protein amino acid sequence was taken from here.
  • Used GeneDesign B2.0 (URL:http://slam.bs.jhmi.edu/gd/) to reverse translate amino acid sequence to DNA sequence and remove restrition site
  • Functional Flexibility of FimH fibrial Adhesin: Insights from a Random Mutant Library. M Schembri, E Sokurenko, P Klemm. Published in Infection and Immunity May 2000, Vol.68, No.5 (URL:http://iai.asm.org/cgi/content/abstract/68/5/2638)
  • Description of FimH:

Bacterial adhesion to target cells enhanced by shear force. Thomas WE, Trintchina E, Forero M, Vogel V, Sokurenko EV. PMID: 12110187